Business

Functional Analyses

Overview of analyses on functional ingredients

Functional analyses

Inquiry about ingredients with biological adjustment effects among farm produces and execution of qualitative and quantitative analyses for the purpose of examination on high-quality plant breeding

Analysis process

It is possible to predict characteristics of plants in a laboratory without a cultivation test as a gene marker related to characteristics of plants (for example: size, color, disease resistance and etc.)

  • 01

    File an application on Seed Industry Center Homepage (http://seedcenter.fact.or.kr) or via a visit

  • 02
    • Manufacture of standard materials
    • Ingredient extraction
    • Separation and filtration of supernatant
  • 03
    • sugar
    • anthocyanin
    • fatty acid
    • carotenoid
    • capsaicinoids
    • glucosinolate
  • 04

    Handling and mailing of a post-analysis report as
    an official document

Analyzed ingredients and analyzers by ingredient

Analyzed ingredients and analyzers by ingredient
Analyzed ingredients Analyzers Characteristics
carotenoid, capsaicinoids and etc. HPLC(PDA)HPLC(PDA)
  • Highly sensitive precision analysis
  • Low-concentration sample analysis
Five types of sugar
(Fructose, glucose, sucrose,
maltose, raffinose)
HPLC(PDA/ELSD)HPLC(PDA/ELSD)
Glucosinolates LC/MS/MSLC/MS/MS simultaneous analyses on various ingredients
Fatty acid aroma components and etc. GC/MSLC/MS/MS data precision,
Highly reliable

High-Perfomance Liquid Chromatograph

고성능 액체 크로마토그래피(ELS) 검출기

Principle of analysis

It is the way to detect to vaporize mobile phase and to make particles of sample to light scattering. It is highly sensitive and fast to stabilize

Feature

  • It is possible to detect various nonvolatile sample than mobile phase
  • Analysis of the sample is possible even though UV detection is difficult due to absent or weak a chromophoric group in substance
  • When using a Refractive Index(RI) detector, a limitation of low detection capability occur. But it is possible to overcome
  • Analysis is possible without complex preprocessing process of sample

Example of analysis

고성능 액체 크로마토그래피(ELS) 검출기

High-Perfomance Mass Spectrometer(LC/MS/MS)

Principle of analysis

  • Triple Quadrupole LC/MS/MS Mass Spectrometer is the method of analysis that make a sample which is separated from Liquid Chromatography(LC) to ionize
  • The ionized sample filters a value of specific ion in Quadrupole(Q1) at first. After that, ions are fragmented in Collision Cell(Q2) secondly. In Quadrupole(Q3) thirdly, the fragmented ions are selectively filtered again and analyze to make possible signal for quantification.

Purpose of use

The mass spectrometer minimizes the effect of the matrix and can analyze up to small amount of concentration through high sensitivity. Thus, it is widely used for quantitative analysis in various fields like agriculture(functional ingredient of crops such as glucosinolate, anthocyanin etc.), environment, organic PoPs etc. in a laboratory and a place on site

Performance and Specifications

  • Scan Speed : 12,000 Da/sec
  • Mass Range (m/z) : 5-2000
  • Scan Types : Full scan MS and selected ion for both Q1 and Q3, Product ion scan precursor ion scan, Neutral loss or gain scan, Multiple reaction monitoring (MRM), Scheduled MRM (sMRM)
  • Minimum MRM Dwell Time : 1 msec
  • Ionization Source : Turbo V™ source housing with TurboionSpray® probe or APCI probe
  • Maximum Temperature : 750℃

Gas Chromatograph-Mass Spectrometer

가스 크로마토그래피(GC) 질량 분석기

Principle of analysis

If the components separated into a single substance in Gas Chromatography are delivered to the Mass Spectrometer Divice (MSD) in sequence, characteristic fragment ion by each component are generated. It is possible to analyze fatty acids, amino acids, the components of scent ect.

In Gas Chromatography(CG),

he sample entered through inlet passes the column in the oven along the flow of the carrier gas. If the sample is separated into a each single substance, it shows the presence of a specific substance as a peak depending on the electrical and chemical properties of each component.In other words, by analyzing in advance the concentrations and substance that the analyst knows, it is possible to estimate the components through the retention time of the substance and measure the amount through the size of the peak of the substance.